Related Experiment Video
Updated: Mar 23, 2026
Study of In Vivo Glucose Metabolism in High-fat Diet-fed Mice Using Oral Glucose Tolerance Test OGTT and Insulin Tolerance Test ITT
Published on: January 7, 2018
Methods for Assessing the In Vivo Role of PTEN in Glucose Homeostasis
Cynthia T Luk1,2, Stephanie A Schroer1, Minna Woo3,4,5,6
1Toronto General Research Institute, University Health Network, Toronto, ON, Canada, M5G 2C4.
Abstract:
PTEN plays an important role in diabetes pathogenesis not only as a key negative regulator of the PI3K/Akt pathway required for insulin action, but also via its role in other cell processes required to maintain metabolic homeostasis. We describe the generation of tissue-specific PTEN knockout mice and models of both type 1 and type 2 diabetes, which we have found useful for the study of diabetes pathogenesis. We also outline common methods suitable for the characterization of glucose homeostasis in rodent models, including techniques to measure beta cell function and insulin sensitivity.
