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Updated: Mar 23, 2026

Capturing Chromosome Conformation Across Length Scales

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

A streamlined tethered chromosome conformation capture protocol.

Idan Gabdank¹, Sreejith Ramakrishnan², Anne M Villeneuve²

¹Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94304, USA.

|April 3, 2016

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A new chromatin capture method rapidly maps genome organization and detects structural variants. This streamlined protocol accelerates the study of nuclear architecture and identifies large-scale DNA rearrangements without cell fractionation.

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

Chromatin conformation capture (3C) techniques map genome spatial organization.
Understanding locus-locus contacts is key to nuclear architecture and function.
Methodological advances are needed to reduce time and material requirements for 3C.

Purpose of the Study:

To develop a modified tethered conformation capture protocol.
To create efficient molecular manipulations for chromatin mapping.
To enable detection of genome rearrangements.

Main Methods:

A modified tethered conformation capture protocol was developed.
The method utilizes rapid and efficient molecular manipulations.
Applied to Caenorhabditis elegans for chromatin interaction mapping.

Keywords:

Caenorhabditis elegans Chromatin Conformation Genome Hi-C TCC

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Last Updated: Mar 23, 2026

Capturing Chromosome Conformation Across Length Scales

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Associated Chromosome Trap for Identifying Long-range DNA Interactions

Published on: April 23, 2011

Main Results:

Generated chromatin interaction maps of Caenorhabditis elegans.
Demonstrated high consistency in chromosome organization across samples.
Detected large-scale structural variants like inversions and translocations.

Conclusions:

The streamlined protocol accelerates chromatin spatial organization mapping.
The method is robust and broadly applicable.
Detects genome rearrangements without cellular or chromatin fractionation.