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Updated: Mar 23, 2026

The Microfluidic Probe: Operation and Use for Localized Surface Processing

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Invited Review Article: Pump-probe microscopy.

Martin C Fischer¹, Jesse W Wilson¹, Francisco E Robles¹

¹Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.

The Review of Scientific Instruments

|April 3, 2016

Summary

This summary is machine-generated.

Multiphoton microscopy uses advanced laser techniques for detailed 3D imaging. Pump-probe microscopy expands capabilities, enabling specific molecular imaging without external labels.

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Last Updated: Mar 23, 2026

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Area of Science:

Biomedical Imaging
Materials Science
Optical Physics

Background:

Multiphoton microscopy offers high-resolution 3D imaging in scattering media.
Current methods (two-photon fluorescence, harmonic generation) have limited target specificity.
Need for advanced techniques to image endogenous molecular targets without labels.

Purpose of the Study:

To provide a tutorial overview of multipulse pump-probe microscopy.
To highlight advancements in laser technology enabling new optical contrasts.
To discuss the potential for enhanced molecular specificity and label-free imaging.

Main Methods:

Review of pump-probe microscopy principles and experimental setups.
Discussion of advancements in fast laser pulse generation, shaping, and detection.
Exploration of multi-color, multi-pulse excitation strategies.

Main Results:

Multipulse excitation offers a vast parameter space for molecular excitation.
Pump-probe microscopy allows tuning of wavelengths, timing, and detection windows.
Potential for significantly greater molecular specificity compared to single-color techniques.

Conclusions:

Multiphoton pump-probe microscopy enables label-free structural and functional imaging.
Advanced laser and detection technologies are key to unlocking new contrast mechanisms.
This technique promises broader applications in biomedical and materials science research.