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Updated: Mar 23, 2026
High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
Published on: April 18, 2021
Liquid Growth of Arrayed Fluorescently Tagged Saccharomyces cerevisiae Strains for Live-Cell High-Throughput
Michael J Cox1, Yolanda T Chong2, Charles Boone1
1The Donnelly Centre, University of Toronto, Toronto, Ontario, M5S 3E1 Canada;
Abstract:
This protocol describes culturing arrays of fluorescently tagged yeast strains to early log-phase in a 96-well format for imaging on a high-throughput (HTP) microscope. The method assumes the use of the synthetic genetic array (SGA) technique to create the array of marked strains. When this approach is coupled with automated image analysis, the subcellular distribution and abundance of tagged proteins can be systematically and quantitatively examined in different genetic backgrounds and/or under different growth regimes.
