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Large Particle Sorting to Isolate Live Parasitic Nematode Eggs.

Alfonso Schmidt1, Tiffany Bouchery1, Graham Le Gros1

  • 1Malaghan Institute of Medical Research, Wellington, New Zealand.

Current Protocols in Cytometry
|April 3, 2016
PubMed
Summary

This study adapted conventional jet-in-air cell sorters to isolate large parasitic nematode eggs. The method successfully separated 60 μm eggs based on light scattering without fluorescent markers.

Keywords:
cell sortingegglarge particlelive parasitenematode

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Area of Science:

  • Parasitology
  • Biotechnology
  • Cell Sorting Technology

Background:

  • Conventional jet-in-air cell sorters are optimized for small particles like 10 μm mammalian lymphocytes.
  • Isolating larger biological entities, such as parasitic nematode eggs, presents unique challenges for these systems.

Purpose of the Study:

  • To evaluate the feasibility of using a standard jet-in-air cell sorter for isolating large parasitic nematode eggs.
  • To outline practical considerations for adapting this technology for larger particle separation.

Main Methods:

  • Utilized a conventional jet-in-air cell sorter equipped with a 200-μm nozzle.
  • Separated Nippostrongylus brasiliensis nematode eggs (up to 60 μm) based on their intrinsic light scattering properties.
  • No fluorescent labeling or molecular markers were employed for separation.

Main Results:

  • Demonstrated successful isolation of large parasitic nematode eggs using a standard cell sorter.
  • Established that light scattering properties are sufficient for differentiating and sorting these larger particles.
  • Confirmed the adaptability of jet-in-air technology for non-mammalian, larger biological samples.

Conclusions:

  • Jet-in-air cell sorting technology can be adapted for isolating large parasitic nematode eggs.
  • Light scattering analysis provides a viable, label-free method for sorting these eggs.
  • This approach offers a practical solution for parasite research and diagnostics.