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Multigrid MALDI mass spectrometry imaging (mMALDI MSI).

Annett Urbanek1,2, Stefan Hölzer1,2, Katrin Knop1,2

  • 1Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstrasse 10, 07743, Jena, Germany.

Analytical and Bioanalytical Chemistry
|April 4, 2016
PubMed
Summary

A new multigrid MALDI MSI technique uses inkjet printing to apply multiple matrices to tissue sections, enabling simultaneous molecular analysis and improving spectral quality without damaging samples.

Keywords:
Inkjet printingMass spectrometry imagingMultigridMultiplexSimultaneous analysisSub-pixel

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Molecular Imaging

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) provides spatially resolved molecular analysis of tissues.
  • Matrix selection significantly impacts MALDI MSI spectral quality.
  • Current methods limit analysis to a single matrix per tissue section, requiring time-consuming and potentially damaging sample manipulation for multiple matrices.

Purpose of the Study:

  • To develop a novel multigrid MALDI MSI (mMALDI MSI) technique for simultaneous application of multiple matrices to a single tissue section.
  • To overcome limitations of conventional MALDI MSI regarding matrix application and sample handling.
  • To enhance the efficiency and quality of spatially resolved molecular analysis in tissues.

Main Methods:

  • Developed an automated inkjet printing system for precise deposition of different matrices onto predefined dot grids on tissue sections.
  • Utilized a cooled printhead to prevent solvent cavitation and a triple-pulse procedure to improve spatial resolution and reduce droplet volume.
  • Investigated direct matrix application to tissue and slide pre-coating methods, followed by MALDI imaging and hematoxylin and eosin (H&E) staining.

Main Results:

  • Successfully demonstrated the mMALDI MSI technique for simultaneous analysis using multiple matrices on one tissue sample.
  • Achieved improved spatial resolution and optimized spectral quality through the prespotting approach.
  • Showcased the compatibility of the method with subsequent H&E staining, avoiding analyte delocalization.

Conclusions:

  • The developed mMALDI MSI technique offers a significant advancement for comprehensive molecular profiling of tissues.
  • Automated inkjet printing enables efficient, high-resolution, and simultaneous multi-matrix application in MALDI MSI.
  • This method enhances analytical capabilities, preserves sample integrity, and facilitates correlative staining for deeper biological insights.