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Shifting the Linear Range in Electrospray Ionization by In-Source Collision-Induced Dissociation
Hideaki Ishii1, Hiroaki Yamaguchi, Nariyasu Mano
1Graduate School of Pharmaceutical Sciences, Tohoku University.
Chemical & Pharmaceutical Bulletin
|April 5, 2016
Summary
In-source collision-induced dissociation (CID) effectively expands the linear range in liquid chromatography-electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS). This method enhances quantitative analysis of compounds with vastly different concentrations.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Chromatography
Background:
- Quantitative analysis using LC/ESI-MS/MS often faces challenges with limited linear dynamic ranges.
- Compounds with significantly different concentrations can be difficult to measure simultaneously.
- Controlling the linear range is crucial for accurate quantification in complex biological samples.
Purpose of the Study:
- To demonstrate the effectiveness of in-source collision-induced dissociation (CID) for adjusting the linear range in LC/ESI-MS/MS.
- To evaluate the impact of manipulating declustering potential on the analytical performance.
- To provide a method for simultaneous quantification of analytes with wide concentration disparities.
Main Methods:
- Utilized in-source collision-induced dissociation (CID) by adjusting the declustering potential in the LC/ESI-MS/MS system.
- Analyzed uracil, deoxyuridine, and uridine to assess the shift in their respective linear ranges.
- Compared the original linear ranges with those obtained after implementing in-source CID.
Main Results:
- Successfully shifted the linear ranges for uracil from 0.3-300 to 10-1000 ng/mL.
- Expanded the linear ranges for deoxyuridine from 1-100 to 30-3000 ng/mL.
- Increased the linear range for uridine from 10-1000 to 100-10000 ng/mL, demonstrating a significant improvement in dynamic range.
Conclusions:
- In-source CID is a valuable technique for optimizing and extending the linear range in LC/ESI-MS/MS.
- This method facilitates the simultaneous quantitative measurement of compounds present at extremely different concentrations.
- The technique offers a practical solution for complex analytical challenges in drug and metabolite analysis.
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