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Modeling single cell antibody excretion on a biosensor.

Ivan Stojanović1, Wolfgang Baumgartner1, Thomas J G van der Velden2

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Summary
This summary is machine-generated.

This study confirms surface plasmon resonance imaging (SPRi) accurately quantifies single hybridoma cell antibody production. The simulation showed over 99% of excreted antibodies were captured, validating SPRi for antibody excretion quantification.

Keywords:
EpCAMHybridoma cellsQuantificationSPRiSimulation

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Area of Science:

  • Biotechnology
  • Biophysics
  • Cell Biology

Background:

  • Accurate quantification of antibody production at the single-cell level is crucial for therapeutic development.
  • Hybridoma cells are widely used for monoclonal antibody production.
  • Surface Plasmon Resonance Imaging (SPRi) is a label-free technique for detecting molecular binding events.

Purpose of the Study:

  • To simulate and validate the use of SPRi for quantifying antibody excretion from single hybridoma cells.
  • To assess the suitability of SPRi for accurate measurement of single-cell antibody production rates.

Main Methods:

  • Computational simulation using Comsol Multiphysics to model antibody excretion and binding.
  • Analysis of antibody diffusion and capture dynamics on an SPRi sensor surface.

Main Results:

  • Simulations demonstrated that antibody loss due to diffusion away from the sensor was less than 1%.
  • Over 99% of excreted antibodies were successfully captured by the SPRi sensor.
  • The SPRi output directly correlated with cellular antibody excretion and binding.

Conclusions:

  • SPRi is a highly suitable and accurate method for quantifying antibody excretion from individual hybridoma cells.
  • The validated model confirms SPRi's capability for precise single-cell antibody production rate determination.
  • This approach offers a powerful tool for optimizing antibody-producing cell lines and therapeutic antibody development.