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Simple fibre optic spectrophotometric cell for pH determination.
S S Besar1, S W Kelly, P A Greenhalgh
1Electronic Engineering Laboratories, University of Kent, Canterbury, UK.
Journal of Biomedical Engineering
|March 1, 1989
Summary
A new, affordable fiber optic spectrophotometric cell for clinical pH measurement is presented. This system offers accurate results using phenol red or universal indicator with either electronic or computer processing.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Spectroscopy
Background:
- Clinical pH monitoring is crucial for patient care.
- Existing spectrophotometric methods can be complex or costly.
- There is a need for simple, inexpensive clinical analytical devices.
Purpose of the Study:
- To develop a simple, inexpensive fiber optic spectrophotometric cell for clinical pH measurements.
- To evaluate the performance of the system using two different indicator reagents and two processing methods.
Main Methods:
- Development of a fiber optic spectrophotometric cell utilizing LED sources and a PIN diode photodetector.
- Utilized plastic optical fiber with a 1.0 mm core diameter.
- Employed phenol red and BDH universal indicator reagents with electronic and computer processing.
Main Results:
- Phenol red with computer processing: 6.0-8.0 pH range, 0.015 pH accuracy.
- Phenol red with electronic processing: 6.8-8.0 pH range, 0.02 pH accuracy.
- BDH universal indicator with computer processing: 5.5-8.5 pH range, 0.05 pH accuracy.
- BDH universal indicator with electronic processing: 6.0-8.0 pH range, 0.03 pH accuracy.
Conclusions:
- The developed fiber optic spectrophotometric cell is a viable, cost-effective tool for clinical pH analysis.
- The system demonstrates good accuracy and a useful measuring range for clinical applications.
- The flexibility in reagent and processing method selection allows for tailored clinical measurements.