Related Experiment Video
Updated: Mar 23, 2026

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells
Sharat Warrier1, Mina Popovic1, Margot Van der Jeught1
1Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, De Pintelaan 185, 9000, Ghent, Belgium.
Abstract:
Mouse embryonic stem cells are known to represent the naïve state of pluripotency, while human embryonic stem cells typically represented the primed state of pluripotency, characterized by a higher drift toward differentiation and some other disadvantages. Here we describe an efficient method for rapid, transgene free induction of the naïve pluripotent state in human by applying a novel combination of small molecules and growth factors in the culture medium (2i, LIF, basic fibroblast growth factor, ascorbic acid, and forskolin). Conversion of primed human embryonic stem cells towards the naive pluripotent state should be confirmed by a detailed characterization of the cells, as described in this chapter.
More Related Videos
07:18A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
Published on: May 12, 2017
10:47Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Published on: October 28, 2011
Related Concept Videos
Maintenance of the ES Cell State
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic...
Induced Pluripotent Stem Cells
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...