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Updated: Mar 23, 2026
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
Protective Effects of Danlou Tablet () against Murine Myocardial Ischemia and Reperfusion Injury In Vivo
Jian-Yong Qi1, Lei Wang1, Dong-Sheng Gu2
1Intensive Care Laboratory, Guangdong Province Hospital of Chinese Medicine, The 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China.
Objective:
To observe the in vivo effect of Danlou Tablet (, DLT) on myocardial ischemia and reperfusion (I/R) injury.
Methods:
DLT effects were evaluated in mouse heart preparation using 30-min coronary occlusion followed by 24-h reperfusion and compared among sham group (n=6), I/R group (n=8), IPC group (ischemia preconditioning, n=6) and DLT group (I/R with DLT pretreatment for 3 days, 750 mg•kg-1•day-1, n=8). The effects of DLT were characterized in infarction size (IS) compared with risk region (RR) and left ventricle using the Evans blue/triphenyltetrazolium chloride double dye staining method in vivo. Furthermore, the dose-dependent effect of DLT on I/R injury was evaluated by double staining method. Five different concentrations of DLT (0.625, 1.25, 2.5, 5 and 10 g•kg-1•day-1) were chosen in this study, and dose-response curve of DLT was obtained on these data.
Results:
The ratio of IS to left ventricle was significantly smaller in the DLT and IPC groups than the I/R group (P<0.05 or P<0.01), the ratio of IS to RR was also reduced in the DLT and IPC groups (P<0.01), while there were no differences in RR among the four groups (P>0.05). Experiments showed incidence of arrhythmias was reduced in the DLT group (P<0.01). Furthermore, DLT produced a dose-dependent inhibitory effect with a half maximal inhibitory concentration of 1.225 g•kg-1•day-1.
Conclusions:
Our research concluded that DLT was effective in reducing I/R injury in mice, and provided experimental supports for the clinical use of DLT.
Insights
Danlou Tablet (DLT) effectively reduces myocardial ischemia and reperfusion (I/R) injury in mice by decreasing infarct size and arrhythmias. This study provides evidence supporting DLT
Area of Science:
- Cardiovascular Research
- Pharmacology
- Traditional Chinese Medicine
Background:
- Myocardial ischemia and reperfusion (I/R) injury is a significant clinical concern.
- Danlou Tablet (DLT) is a traditional Chinese medicine with potential cardioprotective properties.
- Understanding the in vivo effects of DLT on I/R injury is crucial for its clinical application.
Purpose of the Study:
- To investigate the efficacy of Danlou Tablet (DLT) in mitigating myocardial ischemia and reperfusion (I/R) injury in a mouse model.
- To evaluate the dose-dependent effects of DLT on I/R-induced cardiac damage.
- To provide experimental evidence supporting the clinical use of DLT.
Main Methods:
- A mouse model of myocardial I/R injury was established using 30-minute coronary occlusion followed by 24-hour reperfusion.
- DLT was administered to mice for 3 days prior to I/R induction.
- Infarction size (IS) and risk region (RR) were quantified using the Evans blue/triphenyltetrazolium chloride double staining method.
- Arrhythmia incidence was also monitored.
- Dose-response relationships were assessed using varying concentrations of DLT.
Main Results:
- DLT significantly reduced the infarct size (IS) relative to the left ventricle and risk region (RR) compared to the I/R group (P<0.01).
- Ischemic preconditioning (IPC) also demonstrated protective effects, similar to DLT.
- DLT treatment led to a significant reduction in the incidence of arrhythmias (P<0.01).
- DLT exhibited a dose-dependent inhibitory effect on I/R injury, with a half maximal inhibitory concentration (IC50) of 1.225 g•kg⁻¹•day⁻¹.
Conclusions:
- Danlou Tablet (DLT) demonstrates significant cardioprotective effects against myocardial ischemia and reperfusion (I/R) injury in mice.
- DLT effectively reduces infarct size and arrhythmia incidence, supporting its therapeutic potential.
- These findings provide a strong experimental basis for the clinical application of DLT in managing I/R injury.
