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Updated: Mar 23, 2026
Study of Phagolysosome Biogenesis in Live Macrophages
Published on: March 10, 2014
A comparative study of lysosome-targetable pH probes based on phenoxazinium attached with aliphatic and aromatic
Xiu-Li Wang1, Xiao-Jun Li2, Ru Sun1
1College of Chemistry, Chemical Engineering and Material Science, State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, Soochow University, 199 Ren'Ai Road, Suzhou 215123, China. sunru924@hotmail.com ge_jianfeng@hotmail.com.
Abstract:
In this paper, phenoxazinium was used as a fluorophore for the design of pH probes by the photoinduced electron transfer (PET) mechanism. Phenoxazinium with an aliphatic morpholinyl group (probe ) gave increased emission at 665 nm with pH ranging from 7.4 to 4.4; meanwhile, the other one with an aromatic diethylaminophenyl group (probe ) gave nearly OFF-ON emission at 679 nm with pH ranging from 7.4 to 4.2. They both were reversible pH probes with good selectivity. Their optical properties, especially the PET mechanism, were illustrated by (TD)DFT theory. Fluorescence confocal imaging of probes and a typical phenoxazinium dye (Oxazine 1) was also performed, and the results indicated that probes are lysosome-targetable biomarkers.
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