Related Experiment Video
Updated: Mar 23, 2026

Laparoscopic Cholecystectomy with Indocyanine Green Fluorescence: Choledochoscopic Stone Extraction and Primary Duct Suture
Published on: November 25, 2025
Novel and safer endoscopic cholecystectomy using only a flexible endoscope via single port
Hirohito Mori1, Nobuya Kobayashi1, Hideki Kobara1
1Hirohito Mori, Nobuya Kobayashi, Hideki Kobara, Noriko Nishiyama, Shintaro Fujihara, Taiga Chiyo, Maki Ayaki, Tsutomu Masaki, Department of Gastroenterology and Neurology, Kagawa University, Kita, Kagawa 761-0793, Japan.
Aim:
To apply the laparoscopic and endoscopic cooperative surgery concept, we investigated whether endoscopic cholecystectomy could be performed more safely and rapidly via only 1 port or not.
Methods:
Two dogs (11 and 13-mo-old female Beagle) were used in this study. Only 1 blunt port was created, and a flexible endoscope with a tip attachment was inserted between the fundus of gallbladder and liver. After local injection of saline to the gallbladder bed, resection of the gallbladder bed from the liver was performed. After complete resection of the gallbladder bed, the gallbladder was pulled up to resect its neck using the Ring-shaped thread technique. The neck of the gallbladder was cut using scissor forceps. Resected gallbladder was retrieved using endoscopic net forceps via a port.
Results:
The operation times from general anesthetizing with sevoflurane to finishing the closure of the blunt port site were about 50 min and 60 min respectively. The resection times of gallbladder bed were about 15 min and 13 min respectively without liver injury and bleeding at all. Feed were given just after next day of operation, and they had a good appetite. Two dogs are in good health now and no complications for 1 mo after endoscopic cholecystectomy using only a flexible endoscope via one port.
Conclusion:
We are sure of great feasibility of endoscopic cholecystectomy via single port for human.
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