Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Immune Profiling Identifies Inflammatory Signatures in Immune Checkpoint Inhibitor-Related Myocarditis.

JACC. CardioOncology·2026

Same author

Rare twin cysteine residues in the HIV-1 envelope variable region 1 link to neutralization escape and breadth development.

Cell host & microbe·2026

Same author

Targeting PD-1<sup>+</sup> T cells with chimeric antigen receptors to reduce the HIV reservoir.

Science advances·2026

Same author

Soluble CD14 and Lipopolysaccharide-Binding Protein Are Not Superior to Soluble CD25 as Biomarkers for Sarcoidosis.

Diagnostics (Basel, Switzerland)·2026

Same author

HIV controllers, lessons learnt from the lymphoid tissues.

Current opinion in HIV and AIDS·2026

Same author

Dual CD8 and TCR editing in regulatory T cells mediates HLA-A2-restricted tissue-specific homing.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Search research articles

Related Experiment Video

Updated: Mar 23, 2026

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Published on: January 22, 2019

Current approaches to assess HIV-1 persistence.

Riddhima Banga¹, Francesco A Procopio, Matthieu Perreau

¹Division of Immunology and Allergy, Lausanne University Hospital, University of Lausanne, Lausanne, Switzerland.

Current Opinion in HIV and AIDS

|April 8, 2016

Summary

New techniques like digital droplet PCR offer more precise ways to measure HIV reservoirs in patients undergoing treatment. These advances are crucial for evaluating strategies aimed at curing HIV by targeting infected cells.

More Related Videos

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

Related Experiment Videos

Last Updated: Mar 23, 2026

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Published on: January 22, 2019

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

Published on: September 26, 2011

Area of Science:

Virology
Immunology
Molecular Biology

Background:

HIV persistence in CD4 T cells is a major barrier to eradication.
Current methods for quantifying HIV reservoirs have limitations.

Purpose of the Study:

To review recent technical advancements for quantifying HIV reservoirs.
To assess the impact of these new methods on monitoring clinical interventions.

Main Methods:

Digital droplet PCR
Tat/rev-induced limiting dilution analysis
Enhanced quantitative viral outgrowth assay
Whole genome sequencing

Main Results:

These advanced techniques offer increased precision and sensitivity.
They enable better characterization of HIV reservoirs in treated patients.

Conclusions:

New technologies provide powerful tools for assessing interventions against HIV reservoirs.
These methods are vital for advancing HIV cure research.