Whey protein hydrolysates enhance water absorption in the perfused small intestine of anesthetized rats

Kentaro Ito1, Makoto Yamaguchi1, Teruyuki Noma1

  • 1a Food Science Research Laboratories, R&D Division , Meiji Co., Ltd. , Odawara , Japan.

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Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
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