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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Chromatin Structure and RNA Splicing

Chromatin Structure and RNA Splicing

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Related Experiment Video

Updated: Mar 22, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

A Carbohydrate-Derived Splice Modulator.

Sachin Dhar¹, James J La Clair¹, Brian León¹

¹Department of Chemistry and Biochemistry, University of California-San Diego , 9500 Gilman Drive, La Jolla, California 92093-0358, United States.

Journal of the American Chemical Society

|April 9, 2016

Summary

This summary is machine-generated.

New splice modulators offer improved stability for cancer treatment. Researchers developed carbohydrate-based scaffolds for potent and bench-stable splice modulators, overcoming limitations of current compounds.

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Published on: April 26, 2017

Area of Science:

Medicinal Chemistry
Molecular Biology
Oncology

Background:

Small-molecule splice modulators show promise for treating various cancers.
Current splice modulators face challenges due to metabolic instability and off-target effects.
Understanding splice-regulated events is crucial for disease association studies.

Purpose of the Study:

To develop stable and potent splice modulators.
To overcome the limitations of existing splice modulator instability.
To explore carbohydrate motifs as scaffolds for novel splice modulators.

Main Methods:

Adaptation of carbohydrate motifs as a central scaffold.
Synthesis of novel splice modulators.
Evaluation of stability and potency of the developed compounds.

Main Results:

Preparation of bench-stable splice modulators.
Demonstration of rapid access to potent splice modulators.
Carbohydrate scaffolds provide enhanced stability compared to current materials.

Conclusions:

Carbohydrate-based scaffolds offer a promising strategy for developing stable splice modulators.
The newly developed modulators have potential for cancer therapy.
This approach facilitates the discovery of new tools for studying splice regulation.