Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

3.5K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
3.5K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

5.9K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
5.9K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

2.2K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
2.2K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

29.4K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
29.4K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.3K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.3K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.7K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uniaxial order parameters associated with surface SFG spectra. II. Distributions with polar and azimuthal ordering.

The Journal of chemical physics·2026
Same author

Detection of Fentanyl Analogues in Illicit Drug Samples Using Surface-Enhanced Raman Spectroscopy and Random Forest Classification.

Applied spectroscopy·2026
Same author

Carfentanil in the illicit drug supply: A machine learning approach for trace detection using surface-enhanced Raman spectroscopy.

The International journal on drug policy·2026
Same author

Probing interfacial vibrations with IR absorption spectroscopy: from molecular to mesoscopic and macroscopic surfaces.

Faraday discussions·2026
Same author

Heterospectral Principal Component Analysis Two-Dimensional Correlation Spectrosocpy as a Robust Method for Determining Surface Adsorption Preference.

Applied spectroscopy·2026
Same author

Faster, simpler, and more precise calibration curves: Expanding the scope of continuous calibration.

Analytica chimica acta·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 22, 2026

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

10.8K

Surface-Bulk Vibrational Correlation Spectroscopy.

Sandra Roy1, Paul A Covert1, Tasha A Jarisz1

  • 1Department of Chemistry, University of Victoria , Victoria, British Columbia V8W 3V6, Canada.

Analytical Chemistry
|April 9, 2016
PubMed
Summary
This summary is machine-generated.

Correlation analysis combining surface-specific and bulk spectroscopy reveals new details about molecular adsorption. This approach supports competitive binding models and offers insights into adsorption layer transitions.

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.7K
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

11.1K

Related Experiment Videos

Last Updated: Mar 22, 2026

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
07:55

High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis

Published on: September 22, 2017

10.8K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.7K
Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

11.1K

Area of Science:

  • Spectroscopy
  • Surface Science
  • Physical Chemistry

Background:

  • Nonlinear vibrational spectroscopy offers high surface sensitivity by excluding bulk molecules.
  • This technique's specificity can limit its ability to detect concurrent changes in the solution phase.
  • Correlation analysis is a valuable tool for integrating data from complementary spectroscopic methods.

Purpose of the Study:

  • To demonstrate the utility of heterospectral correlation analysis for studying adsorption processes.
  • To reveal details of molecular adsorption by combining surface-specific and bulk spectroscopic techniques.
  • To investigate the adsorption of water and ethanol binary mixtures.

Main Methods:

  • Homo- and heterospectral correlation analysis were applied.
  • Surface-specific nonlinear vibrational spectroscopy was combined with bulk probes (infrared absorption and Raman scattering).
  • The adsorption of water and ethanol binary mixtures was studied as a model system.

Main Results:

  • Correlation analysis between surface-specific and bulk probes provided additional insights into the adsorption process.
  • The results support a competitive binding model for the adsorption of water and ethanol mixtures.
  • New details regarding a dimer-to-bilayer transition during adsorption were uncovered.

Conclusions:

  • Heterospectral correlation analysis enhances the understanding of adsorption phenomena by integrating surface and bulk information.
  • This combined approach provides a more comprehensive view of molecular interactions at interfaces.
  • The study offers new perspectives on competitive adsorption and layer formation mechanisms.