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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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Advance in phage display technology for bioanalysis.

Yuyu Tan1, Tian Tian1, Wenli Liu1

  • 1State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Biotechnology Journal
|April 11, 2016
PubMed
Summary

Phage display is a versatile technology for selecting target-specific ligands like peptides and antibodies. Advances in microfluidic phage display and phage-based biosensors are improving bioanalysis efficiency and throughput.

Keywords:
BiosensorMicrofluidicsPhage displaySelectionSelf-assembly

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Bioengineering

Background:

  • Phage display technology facilitates target gene expression and selection of specific binding ligands.
  • It offers simplicity, high efficiency, and low cost for screening peptides, proteins, and antibodies.
  • Applications span diverse targets from small molecules to cells and tissues.

Purpose of the Study:

  • To review and compare different phage display systems.
  • To highlight microfluidic-based phage display for high-throughput screening.
  • To emphasize advances in phage-based biosensors for bioanalysis.

Main Methods:

  • Review of existing literature on phage display systems.
  • Comparison of various phage display techniques, including microfluidic approaches.
  • Analysis of phage-derived probes and their integration into biosensor platforms.

Main Results:

  • Phage display successfully generates ligands for a wide array of targets.
  • Microfluidic phage display offers enhanced throughput, efficiency, and integration.
  • Phages and their derived ligands are effective as affinity reagents in therapeutics, diagnostics, and biosensors.

Conclusions:

  • Phage display is a powerful tool with broad applications in bioanalysis.
  • Further development of high-throughput methods is crucial for meeting bioanalytical demands.
  • Screening cyclic and functional peptides represents a key future direction in the field.