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Updated: Mar 22, 2026
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
Measuring Redox Status of Melanoma Cells
Feng Liu-Smith1, Tatiana B Krasieva2, Jing Liu3
1Department of Epidemiology, Chao Family Comprehensive Cancer Center, University of California Irvine School of Medicine, B200 Sprague Hall, 839 Health Science Road, Irvine, CA, 92697, USA. liufe@uci.edu.
Abstract:
Redox homeostasis plays multiple roles in essentially all aspects of cellular function, and hence, reliable methods for measuring cellular or tissue redox status are key elements in understanding the redox related signal pathways. However, in the free radical biology field, there are many controversies on the methods to measure reactive oxygen species. In this chapter we describe our experience in measuring superoxide, hydrogen peroxide, and a general redox status using redox-sensitive green fluorescence proteins (roGFPs) in human melanoma cells.
