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A Device for Long-Term Perfusion, Imaging, and Electrical Interfacing of Brain Tissue In vitro.

Nathaniel J Killian¹, Varadraj N Vernekar², Steve M Potter¹

¹Laboratory for NeuroEngineering, Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology Atlanta, GA, USA.

Frontiers in Neuroscience

|April 12, 2016

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This study introduces a new device for long-term culturing of thick brain tissue slices, improving nutrient delivery and waste removal. This innovation supports healthier, more active tissue models for neurobiology research.

Area of Science:

Neurobiology
Tissue Engineering
Electrophysiology

Background:

Thick brain tissue preparations are crucial for in vivo-like studies but suffer from necrosis due to poor nutrient and waste exchange.
Existing electrophysiological tools are inadequate for long-term maintenance of these thick tissue models.

Purpose of the Study:

To develop an integrated device for long-term maintenance and electrophysiological recording of thick, functionally active brain tissue models.
To overcome limitations of current methods hindering days-long experiments with tissue cultures.

Main Methods:

Developed an integrated device featuring interstitial perfusion for nutrient/waste exchange in thick tissue slices.
Utilized perforated multi-electrode arrays for distributed recordings from explanted tissue.

Keywords:

MEA brain slice neurons perforated microelectrode array three-dimensional culture

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Incorporated automated culturing and in situ imaging capabilities.

Main Results:

The device enables long-term maintenance of thick, viable, and functionally active brain tissue models.
Convective perfusion significantly improved tissue viability, evidenced by higher firing rates in perfused vs. unperfused cultures.
The system is economical, easy to assemble, and integrates with standard electrophysiology equipment.

Conclusions:

The novel device facilitates healthier, thicker, and more active tissue cultures for extended experimental periods.
This advancement supports research into normal brain function, neurological disorders, and drug screening, overcoming limitations of acute slice preparations.