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Related Experiment Video

Updated: Mar 22, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

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Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes.

Yu Ting Chow1, Shuxun Chen1, Ran Wang1

  • 1Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, China.

Scientific Reports
|April 13, 2016
PubMed
Summary

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Researchers developed a precise automated microinjection system for single-cell transfection. This technology enables controlled delivery of genetic material, advancing cell modification studies and applications where targeted genetic changes are crucial.

Area of Science:

  • Cell biology
  • Biotechnology
  • Microfluidics

Background:

  • Cell transfection is vital for genetic modification.
  • Single-cell level transfection methods are needed to understand cellular responses.
  • Existing methods may lack precision for specific applications.

Purpose of the Study:

  • To develop an automated, quantitative microinjection technology for precise single-cell transfection.
  • To enable controlled delivery of genetic material into individual cells.
  • To facilitate studies requiring precise genetic modification at the single-cell level.

Main Methods:

  • Developed an automated micropipette-based quantitative microinjection system.
  • Utilized cell pre-patterning in arrays for targeted delivery.

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Related Experiment Videos

Last Updated: Mar 22, 2026

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  • Controlled substance delivery via injection pressure and time.
  • Validated precision using fluorescent dye and quantified expression in human fibroblast cells.

    • Main Results:

    • Achieved precise single-cell microinjection with controlled material delivery.
    • Demonstrated a strong correlation between injected genetic material amount and fluorescence output (GFP, R/G ratio).
    • Successfully transfected human foreskin fibroblast cells with modRNA and plasmids.

    Conclusions:

    • The developed microinjection technique offers precise, automated single-cell transfection.
    • This technology is valuable for challenging transfection scenarios and targeted genetic modification.
    • Enables detailed study of cellular responses to precise genetic alterations.