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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Quantification of protein secondary structure by (13)C solid-state NMR

Fabiana Diuk Andrade1, Lucimara Aparecida Forato1, Rubens Bernardes Filho1

  • 1Embrapa Instrumentação, Rua XV de Novembro 1452, São Carlos, São Paulo, 13560-970, Brazil.

Analytical and Bioanalytical Chemistry
|April 13, 2016
PubMed
Summary

This study introduces a new method using carbon-13 solid-state nuclear magnetic resonance (NMR) and singular value decomposition (SVD) to accurately quantify insoluble protein structures. This technique offers a reliable alternative for analyzing protein secondary structures.

Keywords:
13C solid-state NMRInsoluble proteinProtein secondary structureSingular value decomposition

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