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Related Concept Videos

Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

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Drug concentration is the quantity of a drug present in a biological sample. Measuring drug amounts in biological samples allows the clinician to understand how a drug is absorbed, distributed, metabolized, and excreted. Samples can be obtained through invasive or non-invasive methods. Invasive techniques involve surgical or parenteral interventions to gather blood, cerebrospinal fluid, or tissue biopsy. Conversely, non-invasive approaches provide samples like urine, feces, and saliva.
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Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
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Measurement of Bioavailability: Pharmacokinetic Methods01:30

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Pharmacokinetics is a vital branch of pharmacology that examines how drugs are absorbed, distributed, metabolized, and excreted by the body. Two key methodologies in pharmacokinetics are plasma drug concentration studies and urinary drug excretion analyses, both of which provide critical insights into a drug's therapeutic efficacy and bioavailability.Plasma Drug Concentration-Time StudiesPlasma drug concentration-time studies involve analyzing blood samples at specific intervals to quantify...
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Measurement of Bioavailability: Pharmacodynamic Methods01:20

Measurement of Bioavailability: Pharmacodynamic Methods

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Pharmacodynamic methods provide insights into a drug's effects on physiological processes over time and play a crucial role in understanding bioavailability and therapeutic efficacy. These methods can be broadly classified into acute pharmacological and therapeutic response approaches, each with distinct mechanisms and applications.The acute pharmacological response method directly correlates a drug's physiological effects, such as ECG or pupil diameter changes, to its time course in the body.
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Related Experiment Video

Updated: Mar 22, 2026

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients
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Plasma Voriconazole Estimation by HPLC.

Prerna K Chawla1, Alpa J Dherai2, Tester F Ashavaid2

  • 1Research Laboratories, P.D. Hinduja Hospital and Medical Research Centre, V. S. Marg, Mahim, Mumbai, 400016 India.

Indian Journal of Clinical Biochemistry : IJCB
|April 13, 2016
PubMed
Summary
This summary is machine-generated.

Therapeutic drug monitoring (TDM) of voriconazole, an antifungal, is crucial due to its variable effects. A new HPLC method enables precise measurement of voriconazole plasma levels, improving patient treatment outcomes.

Keywords:
HPLCTherapeutic drug monitoringVoriconazole

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Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Clinical Diagnostics

Background:

  • Voriconazole, a critical antifungal agent, displays significant inter-individual pharmacokinetic variability.
  • This variability necessitates therapeutic drug monitoring (TDM) to optimize patient outcomes and minimize toxicity.

Purpose of the Study:

  • To develop and validate a simple, sensitive, and rapid high-performance liquid chromatography (HPLC) method for quantifying voriconazole in plasma.
  • To establish a reliable analytical tool for routine clinical TDM of voriconazole.

Main Methods:

  • A reversed-phase HPLC method utilizing a C18 column with a mobile phase of acetonitrile and water (7:3) at a flow rate of 1 mL/min.
  • UV detection at 255 nm following plasma protein precipitation with perchloric acid.
  • Method validation included linearity (0.2-15 mg/L) and precision assessment (inter-assay <15%).

Main Results:

  • The developed HPLC method demonstrated a limit of quantification of 0.2 mg/L.
  • The assay proved linear and precise, suitable for clinical application.
  • The method was successfully applied to TDM in 26 patients receiving voriconazole for invasive fungal infections.

Conclusions:

  • The standardized HPLC method provides a robust platform for routine voriconazole TDM.
  • Implementing routine TDM can lead to improved clinical efficacy and reduced adverse events in patients with invasive fungal infections.
  • This analytical approach supports personalized voriconazole dosing strategies.