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Program in BASIC for Ferguson plot analysis, using a personal computer: application to gel electrophoresis in a

E Gombocz1, A Chrambach

  • 1Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.

Electrophoresis
|March 1, 1989
PubMed
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A new BASIC program enables personal computers to perform Ferguson plot analysis for gel electrophoresis. This versatile tool offers objective curve fitting for linear, convex, or concave plots, enhancing accessibility for researchers.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Gel electrophoresis is a fundamental technique in molecular biology.
  • Ferguson plot analysis is crucial for determining molecular size and shape during electrophoresis.
  • Previous analysis methods were often limited to mainframe computers.

Purpose of the Study:

  • To develop a versatile BASIC program for Ferguson plot analysis on personal computers.
  • To enable objective curve fitting for various Ferguson plot types (linear, convex, concave).
  • To make advanced gel electrophoresis data analysis more accessible.

Main Methods:

  • Development of a BASIC program for personal computers.
  • Implementation of polynomial function curve fitting for data analysis.

Related Experiment Videos

  • Application to gel electrophoresis in a single (continuous) buffer system.

    • Main Results:

    • The program provides objective selection of the most appropriate polynomial curve fit.
    • Results are comparable to established methods on mainframe computers.
    • Demonstrates significant versatility in analyzing different Ferguson plot shapes.

    Conclusions:

    • This program facilitates widespread application of Ferguson plot analysis on personal computers.
    • It offers an objective and versatile alternative to traditional analysis methods.
    • Enhances the accessibility of sophisticated gel electrophoresis data interpretation.