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Vertical remission densitometry for the evaluation of nontransparent samples
1Max-Planck-Institut für experimentelle Medizin, Forschungsstelle Neurochemie, Göttingen, Federal Republic of Germany.
Electrophoresis
|March 1, 1989
Summary
Dried gels and blots are unsuitable for transmission densitometry. Remission scanning offers a viable alternative, achieving comparable accuracy and resolution to transmission mode for densitometric evaluation of these samples.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Dried gels and membrane blots are commonly used for sample analysis.
- Traditional densitometric evaluation in transmission mode is hindered by sample opacity.
Purpose of the Study:
- To investigate the efficacy of remission scanning for densitometric evaluation of dried gels and membrane blots.
- To compare the accuracy and resolution of remission scanning with transmission mode.
Main Methods:
- Samples: Gels dried onto filter paper and their blots on membranes.
- Scanning Mode: Remission mode using a computer-controlled high-speed scanning photometer.
- Data Analysis: Digital image processing for spatial resolution and accuracy assessment.
Main Results:
- Remission scanning enables successful densitometric evaluation of nearly nontransparent dried gel and membrane blot samples.
- Data acquisition via computer-controlled scanning photometer and digital image processing achieved spatial resolution and accuracy comparable to transmission mode.
- Signal-to-background ratio in remission mode decreased by 10-30%, influenced by the support material's smoothness.
Conclusions:
- Remission scanning is a suitable method for densitometric analysis of dried gels and membrane blots.
- This technique provides a valuable alternative when transmission mode is not feasible due to sample properties.