Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mirror densitometry for higher sensitivity and resolution.

D Taube1, V Neuhoff

  • 1Max-Planck-Institut für experimentelle Medizin, Forschungstelle Neurochemie, Göttingen, Federal Republic of Germany.

Electrophoresis
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of non-irradiated blood components in Campath (alemtuzumab)-treated renal transplant patients.

Transfusion medicine (Oxford, England)·2016
Same author

A Systematic Review of Outcomes Following Percutaneous Transluminal Angioplasty and Stenting in the Treatment of Transplant Renal Artery Stenosis.

Cardiovascular and interventional radiology·2015
Same author

Regulation of the amino acid availability in the developing brain. No physiological significance of amino acid competition in experimental hyperphenylalaninemia.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience·2014
Same author

Generic tacrolimus in solid organ transplantation.

Clinical transplantation·2014
Same author

Postanastomotic transplant renal artery stenosis: association with de novo class II donor-specific antibodies.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2013
Same author

Microcirculation inflammation associates with outcome in renal transplant patients with de novo donor-specific antibodies.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2012

Placing gels on a mirror for scanning nearly doubles light absorbance. This simple technique enhances sensitivity and resolution in densitometry analysis.

Area of Science:

  • * Gel electrophoresis and densitometry analysis.
  • * Optical imaging techniques.

Background:

  • * Standard densitometry involves light passing through a gel or autoradiogram.
  • * Limited light path can reduce sensitivity and resolution in imaging analysis.

Purpose of the Study:

  • * To enhance the sensitivity and resolution of gel and autoradiogram scanning.
  • * To improve the accuracy of densitometry measurements.

Main Methods:

  • * Utilizing a mirror placed beneath the gel or autoradiogram during scanning.
  • * Performing densitometry in a vertical reflection mode.

Main Results:

  • * Light path within the gel is effectively doubled due to reflection.
  • * Absorbance measurements are nearly doubled compared to standard transmission mode.

Related Experiment Videos

  • * Significant improvements in sensitivity and resolution were observed.

    • Conclusions:

    • * Vertical reflection mode densitometry with a mirror is an effective method to increase sensitivity and resolution.
    • * This technique offers a simple yet powerful enhancement for gel and autoradiogram analysis.
    • * The increased absorbance provides more reliable quantitative data.