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Large-scale purification of active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus
Shinzaburo Takamiya1, Toshihiro Mita2
1Department of Molecular and Cellular Parasitology, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan; Department of Parasitology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.
Parasitology International
|April 14, 2016
Abstract:
A method for purifying active liquid-cultured Caenorhabditis elegans using a modified Baermann apparatus is described. This method consists of two parts: 1) large-scale cultivation of C. elegans in liquid medium and 2) preparation of active nematodes without contamination using the Baermann apparatus.
