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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Mar 22, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Defining Clonal Color in Fluorescent Multi-Clonal Tracking.

Juwell W Wu^1,2, Raphaël Turcotte^1,2, Clemens Alt^1,2

¹Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Scientific Reports

|April 14, 2016

Summary

This summary is machine-generated.

This study introduces a quantitative RGB analysis method for tracking fluorescent melanoma cancer clones. This approach enhances the ability to study clonal expansion and competition in cancer development.

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Published on: October 23, 2019

Area of Science:

Cancer Biology
Genetics
Biotechnology

Background:

Clonal heterogeneity and selection are key drivers of biological processes, including tumor progression.
Combinatorial fluorescent protein expression aids in tracking cell populations and understanding clonal dynamics.
Accurate clonal identity assignment necessitates a validated analytical framework for clonal markings.

Purpose of the Study:

To develop a systematic and quantitative method for analyzing fluorescent melanoma cancer clones.
To enable refined clonal trackability in melanoma research.
To provide a framework for parameterizing and validating clonal markings.

Main Methods:

Development of a quantitative RGB analysis method.
Application of the method to fluorescent melanoma cancer clones.
Demonstration of refined clonal trackability.

Main Results:

A systematic and quantitative RGB analysis method for fluorescent melanoma clones was established.
The method allows for precise parameterization and validation of clonal markings.
Refined clonal trackability of melanoma cells was successfully demonstrated.

Conclusions:

The presented RGB analysis method offers a robust tool for studying clonal dynamics in melanoma.
This quantitative approach facilitates a deeper understanding of clonal expansion and competition in cancer.
The findings advance the utility of fluorescent protein expression for cancer research.