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siRNA targeting YAP gene inhibits gastric carcinoma growth and tumor metastasis in SCID mice

Zhou Zhou1, Jin-Shui Zhu2, Cai-Ping Gao1

  • 1Department of Gastroenterology, Sichuan Provincial People's Hospital, Chengdu, Sichuan 610072, P.R. China.

Oncology Letters
|April 14, 2016
PubMed

Insights

Silencing the Yes-associated protein (YAP) gene significantly reduced gastric cancer growth and metastasis in mice. This approach also promoted tumor cell death and inhibited proliferation and angiogenesis, offering a potential new treatment strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Yes-associated protein (YAP) is frequently activated in various cancers, including gastric carcinoma.
  • Understanding YAP's role is crucial for developing targeted gastric cancer therapies.

Purpose of the Study:

  • To investigate the impact of YAP silencing on gastric cancer progression.
  • To evaluate YAP's role in proliferation, apoptosis, metastasis, and angiogenesis.

Main Methods:

  • Utilized small-hairpin RNA (shRNA) to silence YAP expression in SGC7901 gastric cancer cells.
  • Employed a gastric orthotopic implantation mouse model in severe combined immunodeficiency mice.

Main Results:

  • YAP silencing significantly reduced gastric cancer growth and metastasis.
  • Tumor cell apoptosis was promoted, while proliferation and angiogenesis were inhibited.
  • Downregulation of key genes including TEA domain family member 1, cyclinD1, vascular endothelial growth factor, and fibroblast growth factor-2 was observed.

Conclusions:

  • YAP plays a significant role in gastric cancer proliferation, metastasis, and angiogenesis.
  • RNA interference-mediated YAP silencing presents a promising novel therapeutic strategy for gastric cancer.
Keywords:
RNA interferencegastric carcinomaorthotopic implantation mouse modelyes-associated protein gene

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