Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning.

Area of Science:

• Agricultural Chemistry
• Biotechnology
• Food Science

Background:

• Flaxseed oil extraction is crucial for obtaining valuable omega-3 fatty acids.
• Traditional extraction methods often face challenges with efficiency and oil quality.
• Enzyme-assisted three-phase partitioning (EATPP) offers a novel approach for oil recovery.

Purpose of the Study:

• To optimize enzyme-assisted three-phase partitioning (EATPP) for flaxseed oil extraction.
• To determine the key parameters influencing both the enzymolysis and partitioning stages.
• To evaluate the maximum achievable oil yield and quality using the EATPP method.

Main Methods:

• Flaxseed hydrolysis using optimized enzyme solutions (enzyme type, concentration, temperature, pH).
• Three-phase partitioning (TPP) involving salt and t-butanol addition to crude flaxseed slurry.
• Optimization of t-butanol concentration, salt concentration, and temperature for maximal yield.

Main Results:

• Optimized conditions achieved a maximum flaxseed oil extraction yield of 71.68%.
• Key optimized parameters included 49.29% t-butanol, 30.43% ammonium sulfate, and 35°C.
• The EATPP method demonstrated high efficiency in extracting flaxseed oil.

Conclusions:

• Enzyme-assisted three-phase partitioning (EATPP) is a highly effective method for flaxseed oil extraction.
• The optimized EATPP process yields high extraction rates and maintains oil quality.
• This technique shows significant potential for scalable industrial oil production.