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Related Concept Videos

Vaccine Production01:23

Vaccine Production

30
Vaccine production involves a sequence of upstream and downstream processes to generate a safe and effective immunological product. It begins with cultivating microorganisms, such as viruses or bacteria, to obtain antigenic material. For viral vaccines, mammalian host cells are grown in bioreactors and subsequently infected with the target virus. The virus replicates within the host cells, which are lysed to release viral particles. This lysate is then clarified through filtration or...
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Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
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A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
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Oral Vaccine Development by Molecular Display Methods Using Microbial Cells.

Seiji Shibasaki1, Mitsuyoshi Ueda2

  • 1General Education Center and Graduate School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe, 650-8530, Japan. seiji@huhs.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2016
PubMed
Summary
This summary is machine-generated.

Molecular display technology enables oral vaccine development by presenting antigenic proteins on microbial cells. This approach simplifies vaccine production and administration, offering a convenient alternative to traditional methods.

Keywords:
Candida albicansCandidiasisCell surfaceLactobacillus caseiMolecular displayOral vaccineSaccharomyces cerevisiae

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Area of Science:

  • Biotechnology
  • Vaccinology
  • Microbiology

Background:

  • Oral vaccines offer administration advantages over injectables.
  • Effective vaccines require antigenic proteins combined with adjuvants.
  • Molecular display technology presents a novel approach to vaccine design.

Purpose of the Study:

  • To present methodologies for designing oral vaccines using molecular display technology.
  • To explore the potential of displaying antigenic proteins on microbial cell surfaces.
  • To demonstrate the feasibility of producing oral vaccines with inherent adjuvant properties.

Main Methods:

  • Utilizing molecular display technology to present antigenic proteins on microbial cell surfaces.
  • Employing Saccharomyces cerevisiae and Lactobacillus casei as display systems.
  • Developing oral vaccine candidates against candidiasis.

Main Results:

  • Successful display of antigenic proteins with adjuvant capabilities on microbial cells.
  • Demonstrated convenience in oral vaccine production processes.
  • Development of oral vaccine prototypes against candidiasis.

Conclusions:

  • Molecular display technology provides a convenient platform for oral vaccine production.
  • Microbial cell-surface display offers a promising strategy for oral vaccine development.
  • This technology facilitates the creation of oral vaccines with integrated adjuvant functions.