Related Experiment Video
Updated: Mar 22, 2026
Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Purification of Virus-Like Particles (VLPs) from Plants
Albertha R van Zyl1, Inga I Hitzeroth2
1Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, Private Bag, Rondebosch, 7700, South Africa.
Abstract:
Viral coat proteins expressed in plants often form virus-like particles (VLPs) which are good vaccine candidates as they are safe and highly immunogenic and can be easily purified. The VLPs can be purified by rate-zonal density centrifugation which is based on the size of the VLP or they can be purified by isopycnic centrifugation which is a fast and simple method and results in isolation of VLPs with the same density. Details on how to apply both rate-zonal and isopycnic centrifugation for VLP purification from plants are provided in this chapter.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
06:17Expression and Purification of Virus-like Particles for Vaccination
Published on: June 2, 2016
