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Updated: Mar 22, 2026
Hydrogen Production and Utilization in a Membrane Reactor
Published on: March 10, 2023
Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell
Bat-Erdene Jugder1, Helene Lebhar1, Kondo-Francois Aguey-Zinsou2
1School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052 Australia.
Abstract:
The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, based on the methods previously reported [1], [2], [3], [4]. Our modifications are: •Upstream method optimizations were undertaken on heterotrophic growth media and cell lysis involving ultrasonication.•Two anion exchangers (Q Sepharose and RESOURCE Q) and size exclusion chromatographic (Superdex 200) matrices were successfully employed for purification of a hexameric SH from R. eutropha.•The H2 oxidizing activity of the SH was demonstrated spectrophotometrically in solution and also immobilized on an EPG electrode using cyclic voltammetry.
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