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Updated: Mar 22, 2026
12:59
Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C UV-C Damage
Published on: February 15, 2020
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Hyperoside protects human primary melanocytes against H2O2-induced oxidative damage
Bin Yang1, Qin Yang2, Xin Yang1
1Department of Dermatology, Wuhan General Hospital of Guangzhou Command, Wuhan, Hubei 430070, P.R. China.
Molecular Medicine Reports
|April 16, 2016
Summary
Hyperoside, a compound from Cuscutae semen, protects human melanocytes from oxidative damage. It reduces apoptosis and regulates key signaling pathways, offering potential for vitiligo treatment.
Area of Science:
- Dermatology
- Pharmacology
- Cell Biology
Background:
- Cuscutae semen is recognized in the Chinese Pharmacopoeia for vitiligo treatment.
- The specific mechanisms of its active compounds, like hyperoside, require further investigation.
Purpose of the Study:
- To investigate the protective effects of hyperoside on human melanocytes against oxidative stress.
- To elucidate the underlying molecular mechanisms of hyperoside's action.
Main Methods:
- Cell viability was assessed using the tetrazolium bromide assay.
- A cell model of hydrogen peroxide (H2O2)-induced oxidative damage was utilized.
- Apoptosis, mitochondrial membrane potential, and key protein/mRNA levels (Bcl-2, Bax, caspase 3) were analyzed.
- Signaling pathways including PI3K/AKT and MAPK were examined.
Main Results:
- Hyperoside significantly enhanced human melanocyte viability in a time- and dose-dependent manner.
- Hyperoside pretreatment reduced H2O2-induced melanocyte apoptosis from 54.03% to 17.46%.
- Hyperoside modulated oxidative stress markers, regulated apoptosis-related proteins, and influenced PI3K/AKT and MAPK signaling.
Conclusions:
- Hyperoside demonstrates significant protective effects against oxidative damage in human primary melanocytes.
- These findings suggest hyperoside's potential therapeutic value in conditions involving melanocyte oxidative stress, such as vitiligo.
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