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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

Microarray-Based Phospho-Proteomic Profiling of Complex Biological Systems.

C Rory Goodwin¹, Crystal L Woodard², Xin Zhou³

¹Department of Neurosurgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; Hugo W. Moser Research Institute at Kennedy Krieger, Baltimore, MD, USA.

Translational Oncology

|April 17, 2016

Summary

This summary is machine-generated.

Protein microarrays reveal how PTEN and Akt influence glioma cell signaling. This high-throughput method identifies novel kinase substrates and interactions in tumors, aiding oncogenesis research.

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Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Protein microarrays are established tools for identifying kinase substrates.
Understanding the roles of PTEN and Akt in cancer signaling is crucial.

Purpose of the Study:

To globally interrogate the effects of PTEN and Akt activity on the phospho-kinome in glioma models.
To validate findings in clinical pathological specimens.
To identify novel kinase substrates and interactions in tumor cells.

Main Methods:

Utilized protein microarrays (human kinome chip) to analyze whole cell lysates from glioma tumor samples.
Performed high-throughput profiling of global kinase phosphorylation patterns.
Validated findings in clinical pathological specimens.

Main Results:

Identified global kinase phosphorylation patterns affected by PTEN and Akt activity in glioma.
Discovered novel kinase substrates inherent to tumor cells.
Revealed interactions between tumor cells and the tumor microenvironment.

Conclusions:

Protein microarrays offer a novel, high-throughput method for assessing intracellular signaling events.
This approach is applicable to studying human oncogenesis and other pathophysiologic states.
The findings provide insights into glioma signaling pathways.