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Preparation of monoclonal antibodies against methamphetamine
T Usagawa1, M Nishimura, T Uda
1Research Laboratory, Ube Industries Ltd., Japan.
Journal of Immunological Methods
|April 21, 1989
Abstract:
15 monoclonal antibodies against methamphetamine (MA) were obtained from hybridoma clones by the fusion of mouse 653 myeloma cells with the spleen cells of the immunized mice. The monoclonal antibodies exhibited different cross-reactivities against MA-related compounds and one antibody (MA-15 Ab) was highly specific against MA. A competitive inhibition enzyme-linked immunosorbent assay was established and the detection limit for MA was approximately 20 ng/ml.