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Related Concept Videos

Antibody Structure01:10

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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
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Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Asthma is a chronic respiratory condition for which new therapeutic avenues, including anti-inflammatory drugs like mast cell stabilizers and anti-IgE treatments, continue to be developed.
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Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

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Same author

IgE-class-specific immunosuppression in offspring by administration of anti-IgE to pregnant mice.

The Journal of allergy and clinical immunologyยท2018
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The Way We Walked with Immunology.

Annual review of immunologyยท2018
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Pillars Article: Physico-Chemical Properties of Human Reaginic Antibody: IV. Presence of a Unique Immunoglobulin as a Carrier of Reaginic Activity. J. Immunol. 1966. 97: 75-85.

Journal of immunology (Baltimore, Md. : 1950)ยท2016
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Pillars Article: Physicochemical Properties of Reaginic Antibody: V. Correlation of Reaginic Activity with ฮณE-Globulin Antibody. J. Immunol. 1966. 97: 840-853.

Journal of immunology (Baltimore, Md. : 1950)ยท2016
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Kimishige Ishizaka. Tokyo, Japan.

Chemical immunology and allergyยท2014
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Mast cells and IgE: from history to today.

Allergology international : official journal of the Japanese Society of Allergologyยท2013

Related Experiment Video

Updated: Mar 22, 2026

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
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Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis

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Identification of IgE.

Kimishige Ishizaka1, Teruko Ishizaka1

  • 1La Jolla Institute for Allergy & Immunology, La Jolla, Calif.

The Journal of Allergy and Clinical Immunology
|April 20, 2016
PubMed
Summary

Researchers discovered immunoglobulin E (IgE) as the unique antibody isotype responsible for reaginic hypersensitivity. This finding explains allergic reactions by detailing IgE

Area of Science:

  • Immunology
  • Protein Chemistry
  • Allergy Research

Background:

  • Protein chemistry in the 1950s established that protein biologic activities stem from specific molecular structures.
  • This principle prompted investigation into unique structures of antibodies causing reaginic hypersensitivity reactions.

Observation:

  • Hypothesized that reaginic antibody activity is linked to a distinct immunoglobulin isotype.
  • Experimental animals' immune systems could recognize structural differences in human antibodies.

Findings:

  • Identified immunoglobulin E (IgE) as the unique isotype responsible for reaginic antibody activity.
  • Demonstrated IgE's specific binding to basophils and mast cells via its Fc portion.
  • Showed allergen-induced cross-linking of cell-bound IgE triggers the release of mediators like histamine, initiating allergic reactions.
Keywords:
IgEreagin

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Last Updated: Mar 22, 2026

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
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Implications:

  • Facilitated analysis of the immunologic mechanisms underlying reaginic hypersensitivity.
  • Provided a molecular basis for understanding allergic responses.
  • Opened avenues for targeted therapies against IgE-mediated allergies.