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Lysozyme-loaded lipid-polymer hybrid nanoparticles: preparation, characterization and colloidal stability evaluation
Burcu Devrim1, Aslı Kara2, İmran Vural3
1a Department of Pharmaceutical Technology, Faculty of Pharmacy , Ankara University , Ankara , Turkey ;
Drug Development and Industrial Pharmacy
|April 20, 2016
Summary
Lipid-polymer hybrid nanoparticles (LPNPs) show promise for protein delivery. These nanoparticles, made with poly-ɛ-caprolactone and a lipid shell, effectively encapsulate and release proteins, demonstrating good stability and cellular uptake.
Area of Science:
- Nanotechnology
- Materials Science
- Biomedical Engineering
Background:
- Lipid-polymer hybrid nanoparticles (LPNPs) offer an advanced nanocarrier system, combining features of liposomes and polymeric nanoparticles.
- LPNPs represent a potent alternative for therapeutic delivery applications.
Purpose of the Study:
- To develop and characterize lipid-polymer hybrid nanoparticles (LPNPs) for effective protein delivery.
- To evaluate LPNPs using lysozyme as a model protein.
Main Methods:
- Lysozyme-loaded LPNPs were fabricated using a modified w/o/w double-emulsion-solvent-evaporation technique.
- Poly-ɛ-caprolactone (PCL) formed the nanoparticle core, with a tripalmitin:lecithin mixture creating the lipid shell.
- Characterization included particle size, zeta potential, morphology, encapsulation efficiency, in vitro release, stability, and cytotoxicity studies.
Main Results:
- LPNPs exhibited particle sizes ranging from 58.04 to 2009.00 nm and spherical morphology.
- Protein loading capacity varied from 5.81% to 60.32%, with biphasic release (burst followed by sustained).
- LPNPs showed good colloidal stability in various media and significant uptake (83.3%) by L929 cells.
Conclusions:
- LPNPs formulated with PCL core and tripalmitin:lecithin lipid shell are a promising platform for protein delivery.
- The developed LPNPs demonstrate favorable characteristics for therapeutic applications.
