Related Experiment Video
Updated: Mar 22, 2026
11:02
Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
Published on: October 30, 2013
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MUC1 stimulates EGFR expression and function in endometrial cancer
Brian J Engel1, Jessica L Bowser2, Russell R Broaddus3
1Department of BioSciences, Rice University, Houston, TX 77005, USA.
Oncotarget
|April 20, 2016
Summary
MUC1 protein drives epidermal growth factor receptor (EGFR) expression and signaling in endometrial cancer. Targeting both MUC1 and EGFR may improve treatment for advanced tumors.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Endometrial cancer lacks effective treatments for advanced stages.
- MUC1 protein is involved in cell signaling, including via the epidermal growth factor receptor (EGFR).
Purpose of the Study:
- To investigate the role of MUC1 in regulating EGFR expression and function in endometrial cancer.
- To explore the therapeutic potential of targeting the MUC1-EGFR pathway.
Main Methods:
- Utilized siRNA knockdown and CRISPR/Cas knockout to deplete MUC1.
- Analyzed EGFR gene expression, mRNA, protein levels, and signaling pathways.
- Performed MUC1 binding assays to EGFR promoter regions.
- Assessed cell proliferation and survival in 2D and 3D cultures.
- Evaluated sensitivity to the EGFR inhibitor lapatinib.
- Correlated MUC1 and EGFR expression with tumor proliferation in patient samples.
Main Results:
- MUC1 depletion significantly reduced EGFR expression, protein levels, and downstream signaling.
- MUC1 directly bound to the EGFR promoter, influencing its transcription.
- MUC1 knockout suppressed EGFR-dependent cell proliferation and enhanced sensitivity to lapatinib.
- Co-expression of MUC1 and EGFR correlated with increased proliferation in human endometrial tumors.
Conclusions:
- MUC1 is a key regulator of EGFR expression and signaling in endometrial cancer.
- Dual targeting of MUC1 and EGFR presents a promising therapeutic strategy for endometrial tumors.
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