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New streptophenazines from marine Streptomyces sp. 182SMLY
Ying Liang1, Lu Chen1, Xuewei Ye1
1a Ocean College, Zhoushan Campus , Zhejiang University , Zhoushan , China.
Natural Product Research
|April 22, 2016
Summary
Marine actinomycetes yielded six phenazines, including three novel compounds. One new phenazine, (-)-streptophenazine B, demonstrated potent antibacterial activity against methicillin-resistant Staphylococcus aureus.
Area of Science:
- Marine microbiology
- Natural product chemistry
- Pharmacology
Background:
- Marine actinomycetes are a rich source of bioactive natural products.
- Phenazines are a class of compounds with diverse biological activities.
- Antibiotic resistance, particularly from methicillin-resistant Staphylococcus aureus (MRSA), is a significant global health concern.
Purpose of the Study:
- To isolate and characterize phenazine compounds from a marine actinomycete.
- To evaluate the antibacterial activity of the isolated compounds against MRSA.
Main Methods:
- Isolation of compounds using chromatographic techniques.
- Structure elucidation using Nuclear Magnetic Resonance (NMR), High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS), optical rotation, and Circular Dichroism (CD) data.
- Determination of Minimum Inhibitory Concentration (MIC) to assess antibacterial activity.
Main Results:
- Six phenazines were isolated from Streptomyces sp. 182SMLY.
- Three new phenazines, designated (-)-streptophenazines M-O, were identified.
- Known phenazines 1-carbomethoxyphenazine and (-)-streptophenazines A and B were also isolated.
- (-)-Streptophenazine B exhibited significant inhibitory activity against MRSA with a MIC value of 4.2 μg/mL.
Conclusions:
- The marine actinomycete Streptomyces sp. 182SMLY produces a variety of phenazine derivatives.
- (-)-Streptophenazine B is a promising lead compound for the development of new agents to combat MRSA infections.
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