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New artificial fluoro-cofactor of hydride transfer with novel fluorescence assay for redox biocatalysis
Lei Zhang1, Jun Yuan, Yufang Xu
1Shanghai Key Laboratory of Chemical Biology, State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. yfxu@ecust.edu.cn.
Chemical Communications (Cambridge, England)
|April 22, 2016
Abstract:
A new artificial fluoro-cofactor was developed for the replacement of natural cofactors NAD(P), exhibiting a high hydride transfer ability. More importantly, we established a new and fast screening method for the evaluation of the properties of artificial cofactors based on the fluorescence assay and visible color change.
