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Related Concept Videos

Genetic Screens02:46

Genetic Screens

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
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Competitive Genomic Screens of Barcoded Yeast Libraries
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A Microfluidic Platform for High-Throughput Screening of Small Mutant Libraries.

Ji Won Lim1, Kwang Soo Shin1, Jaemin Moon1

  • 1Department of Biomedical Engineering, ‡Department of Mechanical Engineering, and §Department of Chemical Engineering, Ulsan National Institute of Science and Technology , 50 UNIST-gil, Ulsan 44919, Republic of Korea.

Analytical Chemistry
|April 23, 2016
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Summary

We developed a novel microfluidic high-throughput screening (HTS) platform for isolating microorganisms. This advanced system overcomes limitations of conventional methods, enabling efficient screening for synthetic biology and metabolic engineering applications.

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Area of Science:

  • Microbiology
  • Synthetic Biology
  • Metabolic Engineering
  • Microfluidics

Background:

  • Screening and isolating microorganisms from mutated recombinant libraries is vital for advancing synthetic biology and metabolic engineering.
  • Conventional screening tools often face limitations in throughput, cost, and labor.
  • There is a need for more efficient and scalable screening platforms.

Purpose of the Study:

  • To introduce a novel microfluidic high-throughput screening (HTS) platform designed for efficient microorganism isolation.
  • To demonstrate the platform's capability in handling long-term cell culture and high-throughput screening.
  • To showcase the platform's versatility through reporter gene expression and cellular growth rate difference assays.

Main Methods:

  • Development of a microfluidic platform utilizing a fluid array for compartmentalizing bacterial cells in microwells.
  • Implementation of a capillary-driven sample relocation method for extracting selected cells.
  • Demonstration of two HTS methods: reporter gene expression levels and cellular growth rate differences.

Main Results:

  • The platform successfully compartmentalized bacterial cells and enabled long-term culture with high throughput.
  • Reporter gene-based HTS demonstrated successful screening and isolation of target cells from mixed populations.
  • The platform effectively screened and sorted cells based on cellular growth rate differences, a challenging task for conventional methods.

Conclusions:

  • The novel microfluidic HTS platform offers significant advantages over conventional screening tools.
  • The platform is versatile and can be applied to various microbiological assays, including metabolite detection and microbial biosensors.
  • This technology represents a significant advancement for high-throughput screening systems in microbiology and biotechnology.