Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

7.4K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experience-dependent critical period for synaptic plasticity in the rat olfactory amygdala.

Frontiers in synaptic neuroscience·2026
Same author

A transfer triage tool for COVID-19 mass critical care surges.

Scientific reports·2025
Same author

Oncologic Considerations for Primary Facial Reanimation Following Parotid Adenoid Cystic Carcinoma Resection.

Microsurgery·2024
Same author

Free Flap Abdominal Wall Reconstruction: A Review.

Eplasty·2024
Same author

Mechanical force activates the light-dependent channels TRP and TRPL in excised patches from the rhabdomere of Drosophila photoreceptors.

Neuroscience·2024
Same author

Transient Synaptic Enhancement Triggered by Exogenously Supplied Monocarboxylate in Drosophila Motoneuron Synapse.

Neuroscience·2024

Related Experiment Video

Updated: Mar 22, 2026

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

13.3K

Single Ca(2+)-activated Cl(-) channel currents recorded from toad olfactory cilia.

Ricardo Delgado1, Casilda V Mura1, Juan Bacigalupo2

  • 1Department of Biology, Faculty of Sciences, University of Chile, 7800024, Ñuñoa, Santiago, Chile.

BMC Neuroscience
|April 27, 2016
PubMed
Summary

Researchers identified novel calcium-activated chloride channels in toad olfactory cilia, suggesting a new role in odor transduction beyond known channels like ANO2. These findings advance our understanding of olfactory sensory neuron function.

Keywords:
Anoctamin-2Bestrophin-2CalciumClCa4lIon channelOdor transductionOlfactory ciliaOlfactory sensory neuron

More Related Videos

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
10:53

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Published on: July 3, 2013

21.1K
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
10:16

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

27.5K

Related Experiment Videos

Last Updated: Mar 22, 2026

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

13.3K
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
10:53

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Published on: July 3, 2013

21.1K
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
10:16

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

Published on: July 13, 2015

27.5K

Area of Science:

  • Olfactory neuroscience
  • Ion channel physiology
  • Chemosensation

Background:

  • Odor transduction involves cyclic adenosine monophosphate (cAMP) signaling and cyclic nucleotide-gated (CNG) channels in olfactory sensory neurons.
  • Calcium influx through CNG channels activates chloride channels, enhancing neuronal depolarization.
  • Anoctamin 2 (ANO2) is a primary calcium-activated chloride channel in olfaction, but its absence in knockout mice only partially affects olfactory responses, suggesting other channels are involved.

Purpose of the Study:

  • To investigate the presence and function of calcium-activated chloride channels in toad olfactory cilia.
  • To identify potential novel channels involved in olfactory transduction beyond ANO2.

Main Methods:

  • Recording of unitary chloride currents from inside-out membrane patches excised from individual toad olfactory cilia.
  • Characterization of channel conductance and calcium dependence.
  • Immunoreactivity analysis using antibodies against ClCa and ANO2 channels.

Main Results:

  • Detection of unitary chloride currents with significant calcium dependence in toad olfactory cilia.
  • Identified conductances of 12 and 24 pS, which are higher than previously reported channels.
  • Observed approximately fivefold higher calcium affinity (K0.5 = 0.38 µM) compared to other channels.
  • Found immunoreactivity for both anti-ClCa and anti-ANO2 antibodies in olfactory cilia.

Conclusions:

  • The findings suggest the presence of a novel olfactory cilia channel with a significant role in odor transduction.
  • The results are consistent with a cooperative function of ClCa and ANO2 channels in olfactory chemotransduction.