Related Experiment Video
Updated: Sep 5, 2026

Metabolomic Analysis of Rat Brain by High Resolution Nuclear Magnetic Resonance Spectroscopy of Tissue Extracts
Published on: September 21, 2014
Indirect determination of tungstate in rat tissues by atomic absorption spectrometry
Abstract:
An indirect method is described for the determination of tungsten as tungstate in tissue samples by atomic absorption spectrometry (AAS). Tungstate forms a stable ion-association complex [Fe(dipy)3]2+WO4(2-) (dipy = 2,2'-dipyridyl) in acidic solution, which can be extracted into chloroform with an efficiency of higher than 98%. The extract can be analysed for iron (and hence indirectly for WO4(2-] by flame AAS after stripping back into 60% perchloric acid. The calibration graph is linear up to 19 p.p.m. of WO4(2-) and the limit of detection is 0.17 p.p.m. Many foreign ions do not interfere and the method has been applied successfully to the determination of tungstate in rattus norvegicus tissue samples.
More Related Videos
Related Concept Videos
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview
The ATR process begins by directing a beam...
Atomic Absorption Spectroscopy: Overview
When irradiated by EMR of a particular wavelength, these...
Atomic Absorption Spectroscopy: Instrumentation
The atomizer used in AAS can be either a flame atomizer or an...
Atomic Absorption Spectroscopy: Atomization Methods
Atomic Absorption Spectroscopy: Interference
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

