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Updated: Mar 22, 2026
Development of a Novel Internal Fixation Model for Rat Radial Fractures: Fracture Healing Assessment and Dorsal Root Ganglion Isolation
Published on: March 13, 2026
Culture-Negative Infection After Operative Fixation of Fractures
Ida L Gitajn1, Marilyn Heng, Michael J Weaver
1*Department of Orthopaedic Surgery, Harvard Combined Orthopaedics Residency Program, Boston, MA; †Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School Orthopedic Trauma Initiative, Boston, MA; and ‡Department of Orthopedic Surgery, Brigham and Women's Hospital, Harvard Medical School Orthopedic Trauma Initiative, Boston, MA.
Objectives:
(1) Compare the outcomes of patients with orthopaedic trauma with culture-negative infection with those with pathogens identified; (2) identify the incidence of culture-negative infection and describe the common characteristics.
Design:
Retrospective study.
Setting:
Two level 1 trauma centers.
Patients:
A total of 391 patients 16 years of age or older who underwent irrigation and debridement for surgical site infection after having undergone fracture fixation were included.
Intervention:
Patients underwent irrigation and debridement with cultures, and antibiotic therapy was initiated.
Main Outcome Measurement:
Treatment failure due to unsuccessful eradication of infection and time to union.
Results:
We found 9% incidence of culture-negative infection. Approximately one-third of patients in both groups went on to have treatment failure (25% of pathogen-specific infections, 38% of culture-negative infections, P = 0.15), and there was no difference between the 2 groups with regard to time to union (22 vs. 24 weeks, P = 0.55). More than one-third of patients required subsequent reconstructive procedure and 5% of patients in each group required amputation to control their infection. There was no difference between the groups with respect to the use of antibiotics before intervention and culture.
Conclusion:
This study confirms the devastating effect that postoperative infections can have and suggests that, with clinical sign of infection, negative cultures do not portend a better prognosis. These entities should be treated in a similar manner to infections with positive cultures. Furthermore, we believe that future studies should not strictly rely on the presence of positive intraoperative cultures. Consensus as to what constitutes a clinical infection, in the absence of positive cultures, is needed.
Level Of Evidence:
Prognostic Level III. See Instructions for Authors for a complete description of levels of evidence.
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