Related Experiment Video
Updated: Mar 22, 2026
08:06
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
9.6K
Single-domain antibody based thermally stable electrochemical immunosensor
Aparajita Singh1, Syed Khalid Pasha1, Pandiaraj Manickam1
1BioMEMS and Microsystems Laboratory, Department of Electrical and Computer Engineering, Florida International University, Miami, FL 33174, United States.
Biosensors & Bioelectronics
|April 30, 2016
Summary
This study developed a thermally stable electrochemical immunosensor using single-domain antibodies (sdAbs) for Ricin detection. These novel sensors offer improved stability and shelf-life in harsh environments, enabling portable point-of-care diagnostics.
Area of Science:
- Electrochemistry
- Biosensors
- Immunotechnology
Background:
- Conventional antibodies in electrochemical immunosensors are sensitive to environmental factors, limiting their use in harsh conditions.
- Ricin Chain-A, a potent toxin, serves as a model analyte for developing robust detection systems.
Purpose of the Study:
- To fabricate a thermally stable electrochemical immunosensor for Ricin detection.
- To evaluate the performance of single-domain antibodies (sdAbs) as recognition elements in harsh environments.
- To compare sdAb-based immunosensors with conventional antibody-based sensors.
Main Methods:
- Fabrication of electrochemical immunosensors using single-domain antibodies (sdAbs) and conventional antibodies.
- Detection of Ricin Chain-A using cyclic voltammetry.
- Assessment of sensor stability and shelf-life at elevated temperatures (up to 40°C).
Main Results:
- The sdAb-based immunosensor detected Ricin in a linear range of 1log(fg/mL)-1log(μg/mL) with a sensitivity of 0.07μA/log(g/mL)/cm(2).
- Miniaturized sensors demonstrated enhanced shelf-life and stability at temperatures up to 40°C.
- sdAb immunosensors showed superior performance compared to conventional antibody-based sensors in terms of thermal stability.
Conclusions:
- Single-domain antibodies enable the development of thermally stable electrochemical immunosensors.
- These sensors are suitable for detecting Ricin in demanding environmental conditions.
- The developed technology facilitates portable, point-of-care immunosensing applications.
