Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prediction of Articular Cartilage Markers with Multispectral Imaging.

IEEE transactions on medical imaging·2026

Same author

Early Post‑Traumatic Osteoarthritis Features Greater Cartilage Extracellular Matrix Proteoglycan Loss Compared to the Pericellular Matrix.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026

Same author

High Median Nerve Reconstruction with Vascularized Ulnar Nerve and Extensor Carpi Radialis Brevis Motor Branch to Anterior Interosseous Nerve Transfers.

Plastic and reconstructive surgery. Global open·2026

Same author

Electromyography Frequency Spectrum Is a Better Indicator of Sleep Bruxism Severity Related to Temporomandibular Disorder Pain Than Conventional Masticatory Muscle Activation and Bruxism Time Indices-A Pilot Study.

Journal of sleep research·2026

Same author

Association of major adverse cardiovascular events with the apnea-hypopnea index, desaturation severity parameters, and cardiac troponins in participants of the Akershus Sleep APnea (ASAP) cohort.

Sleep·2026

Same author

Ultrasmall Surface-Charge-Modified Tantalum Oxide Nanoparticles for the Assessment of Articular Cartilage Using Contrast-Enhanced Computed Tomography.

ACS nano·2026

Same journal

Pulsatile Hemodynamics of Prehypertension and Hypertension: Associations with Pressure and Sex.

Annals of biomedical engineering·2026

Same journal

A Pressure Difference-Based Strategy for Blood Oxygen Control in Membrane Oxygenators: Reduced Modeling, Computational Simulation, and Exploratory In Vivo Evaluation.

Annals of biomedical engineering·2026

Same journal

Multidirectional Optical Bone Densitometry Using a Simulation-Based Machine Learning Model: Experimental Validation with Bone Phantoms.

Annals of biomedical engineering·2026

Same journal

Numerical Study of Human Torso Mechanical Response and Injury Assessment Under Blast Loading with Bulletproof Protection.

Annals of biomedical engineering·2026

Same journal

Immediate and Mid-Long-Term Effects of Foot Orthoses on Gait Biomechanics and Clinical Characteristics in Medial Knee Osteoarthritis: A Systematic Review and Meta-analysis.

Annals of biomedical engineering·2026

Same journal

Screening and Evaluation of Post-stroke Dysphagia: Insights from Neurology, Artificial Intelligence and Data Science-A Scoping Review.

Annals of biomedical engineering·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 22, 2026

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

Published on: April 23, 2017

Cationic Contrast Agent Diffusion Differs Between Cartilage and Meniscus.

Juuso T J Honkanen^1,2, Mikael J Turunen³, Jonathan D Freedman⁴

¹Department of Applied Physics, University of Eastern Finland, POB 1627, 70211, Kuopio, Finland. juuso.honkanen@uef.fi.

Annals of Biomedical Engineering

|May 1, 2016

Summary

This summary is machine-generated.

Diffusion of contrast agents differs between cartilage and meniscus. This study reveals distinct diffusion kinematics in these knee joint tissues, impacting contrast-enhanced computed tomography (CECT) imaging interpretation.

Keywords:

Computed tomography Contrast enhancement Diffusion flux Diffusion kinematics Normalized attenuation

More Related Videos

Characterization of Intra-Cartilage Transport Properties of Cationic Peptide Carriers

Characterization of Intra-Cartilage Transport Properties of Cationic Peptide Carriers

Published on: August 10, 2020

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Published on: February 8, 2022

Related Experiment Videos

Last Updated: Mar 22, 2026

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

An Experimental and Finite Element Protocol to Investigate the Transport of Neutral and Charged Solutes across Articular Cartilage

Published on: April 23, 2017

Characterization of Intra-Cartilage Transport Properties of Cationic Peptide Carriers

Characterization of Intra-Cartilage Transport Properties of Cationic Peptide Carriers

Published on: August 10, 2020

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Published on: February 8, 2022

Area of Science:

Biomedical Imaging
Biomaterials Science
Musculoskeletal Research

Background:

Contrast-enhanced computed tomography (CECT) is crucial for assessing articular cartilage and meniscus composition and structure.
Tissue-specific differences in structure and composition may influence contrast agent diffusion and distribution.
Understanding these variations is key for accurate CECT image interpretation.

Purpose of the Study:

To investigate the diffusion kinematics of a novel iodine-based cationic contrast agent (CA(2+)) in articular cartilage and meniscus.
To compare the diffusion behavior of the contrast agent between cartilage and meniscus.
To assess the impact of tissue-specific diffusion on CECT imaging.

Main Methods:

Harvesting cylindrical cartilage and meniscus samples from bovine knee joints.
Immersing samples in a cationic contrast agent and imaging using micro-CT over 48 hours.
Quantifying normalized X-ray attenuation, contrast agent diffusion flux, and tissue composition (water, collagen, proteoglycan).

Main Results:

Contrast agent distribution patterns differed significantly between cartilage and meniscus.
Normalized attenuation and diffusion flux were notably higher in cartilage compared to meniscus (p < 0.05).
These findings indicate distinct diffusion properties inherent to each tissue type.

Conclusions:

Diffusion kinematics of contrast agents vary significantly between articular cartilage and meniscus.
These tissue-specific variations must be considered for accurate interpretation of CECT images, especially when evaluating both tissues concurrently.
This research provides essential insights for optimizing CECT protocols and diagnostic accuracy in knee joint assessments.