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Updated: Mar 21, 2026
08:32
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
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The endothelial nitric oxide synthase (eNOS) polymorphism in otitis media with effusion (OME)
M Ates1, C Cevik1, R Dokuyucu2
1Mustafa Kemal University, School of Medicine, Department of Otorhinolaryngology, Hatay, Turkey.
Summary
This study found that the G allele of the endothelial nitric oxide synthase (eNOS) G894T polymorphism may predispose children to otitis media with effusion (OME). This genetic link is a novel finding in Turkish pediatric patients with OME.
Area of Science:
- Genetics
- Pediatrics
- Otolaryngology
Background:
- Otitis media with effusion (OME) is a common childhood condition following upper respiratory tract infections.
- Nitric oxide (NO) plays a role in hearing loss, but the specific involvement of endothelial nitric oxide synthase (eNOS) polymorphisms in OME is not well-understood.
Purpose of the Study:
- To investigate the association between eNOS gene polymorphisms and OME in pediatric patients.
- To determine if specific eNOS variants contribute to the development of OME in children.
Main Methods:
- A case-control study involving 89 pediatric OME patients and 85 healthy controls.
- DNA analysis using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with BanII enzyme.
- Comprehensive ENT and audiological examinations were performed on all participants.
Main Results:
- No significant difference in genotype distributions (G/G, G/T, T/T) of eNOS Glu298Asp polymorphism between OME patients and controls.
- A significant association was found when comparing allele distributions between the patient and control groups (P=0.037).
Conclusions:
- The G allele of the eNOS G894T polymorphism is identified as a potential predisposing factor for OME development in children.
- This study provides the first evidence in Turkey correlating eNOS G894T polymorphism with OME in pediatric cases.
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