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Updated: Mar 21, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Published on: May 7, 2018
Stereochemical Study of Sphingosine by Vibrational Circular Dichroism
Atsufumi Nakahashi1, Ananda Kumar C Siddegowda1, Mostafa A S Hammam1
1Faculty of Advanced Life Science, Frontier Research Center for the Post-Genome Science and Technology, Hokkaido University , Kita 21, Nishi 11, Sapporo 001-0021, Japan.
Abstract:
Vibrational circular dichroism (VCD) was first applied to the stereochemical analysis of sphingosine. VCD patterns derived from the C═C stretch as well as other mid-infrared (IR) regions were practical markers to discriminate all the stereoisomers of intact sphingosine. Glutaraldehyde was found as an excellent derivatizing reagent for sphingosine which improves its solubility in VCD-friendly nonpolar solvents such as chloroform and enhances the VCD intensities by forming a rigid cyclized structure.
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