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Updated: Jun 27, 2026

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
Published on: August 21, 2009
Lipidomic Insight Across 48 Saccharomyces cerevisiae Wine Yeast Strains Revealed Novel N-Acyl
Md Abdul Malek1, Jayashankar Jayaprakash1, Kevin S Huynh1
1Graduate School of Global Food Resources, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo 060-0809, Japan.
Abstract:
Saccharomyces cerevisiae (SC), an important unicellular yeast species in the biotechnology, beverage, and food industries, has remarkable applications in ethanol production. Lipids in SC play a crucial role in stress management during the fermentation process. Despite the importance of SC yeasts, comprehensive lipid profiling across multiple yeast strains remains limited. This study aimed to elucidate the comprehensive lipidomic profiles of 48 wine yeast strains of the SC species using a nontargeted liquid chromatography-mass spectrometry approach. A total of 135 lipid species were identified, representing five major lipid categories. Among the SC strains, strain 19 contained the highest relative concentrations of α-linolenic acid (ω-3) and linoleic acid (ω-6). Levels of polyunsaturated fatty acids were 4.6-fold higher in SC46 compared to SC13, suggesting strain-specific variations. Glycerolipids were the most abundant lipid components, with triacylglycerols showing the highest relative abundance in SC10, SC41, and SC48. These findings should be interpreted in light of the semi-quantitative nature of the dataset. Interestingly, bioactive lipids, such as N-acyl lysophosphatidylethanolamines, were putatively identified and structurally characterized using high-resolution mass spectra and MS/MS fragmentation patterns. This study provides insight into strain-specific differences in the yeast lipidome and may help improve our understanding of fermentation kinetics and industrial wine quality.
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